Review



bmpr2 ecd fc fusion  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Sino Biological bmpr2 ecd fc fusion
    Antibody 3F6 reduces <t>BMPR2-ECD</t> ligand-binding activity in a modified immunoprecipitation assay. Neutralizing the ligand-binding activity of BMPR2-ECD using various amounts of 3F6. Results are quantified by ELISA and expressed as mean ± SEM relative to the ligand-binding activity of BMPR2-ECD in the absence of 3F6. n ≥ 3 per condition. Asterisk indicates p < 0.05 by paired t test
    Bmpr2 Ecd Fc Fusion, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmpr2+ecd+fc+fusion/Human+BMPR2+%2F+BMPR-II+Protein/pmc06558810-25-0-2
    Average 93 stars, based on 2 article reviews
    bmpr2 ecd fc fusion - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Identification of a bone morphogenetic protein type 2 receptor neutralizing antibody"

    Article Title: Identification of a bone morphogenetic protein type 2 receptor neutralizing antibody

    Journal: BMC Research Notes

    doi: 10.1186/s13104-019-4367-0

    Antibody 3F6 reduces BMPR2-ECD ligand-binding activity in a modified immunoprecipitation assay. Neutralizing the ligand-binding activity of BMPR2-ECD using various amounts of 3F6. Results are quantified by ELISA and expressed as mean ± SEM relative to the ligand-binding activity of BMPR2-ECD in the absence of 3F6. n ≥ 3 per condition. Asterisk indicates p < 0.05 by paired t test
    Figure Legend Snippet: Antibody 3F6 reduces BMPR2-ECD ligand-binding activity in a modified immunoprecipitation assay. Neutralizing the ligand-binding activity of BMPR2-ECD using various amounts of 3F6. Results are quantified by ELISA and expressed as mean ± SEM relative to the ligand-binding activity of BMPR2-ECD in the absence of 3F6. n ≥ 3 per condition. Asterisk indicates p < 0.05 by paired t test

    Techniques Used: Ligand Binding Assay, Activity Assay, Modification, Immunoprecipitation, Enzyme-linked Immunosorbent Assay

    Antibody 3F6 reduces activation of BMP pathway in HEK293T cells. a Expression of endogenous BMPR2 by HEK293T cells compared to β-actin loading control. Approximate molecular weights are indicated. b , c BMP2 induces phosphorylation of SMAD1, 5, and 8 (pSMAD1,5,8) in HEK293T cells and this response is blunted by pre-treatment with 3F6. Approximate molecular weights are indicated in b . Results are quantified in c and expressed as mean ± SEM ratio of phosphorylated SMAD1,5,8: total SMAD1 relative to BMP2 treatment alone. No effect on BMP2-responsiveness was observed with pre-treatment using control ascites (Ctrl Asc.). n = 3 per condition. Asterisk indicates p < 0.05 by paired t test
    Figure Legend Snippet: Antibody 3F6 reduces activation of BMP pathway in HEK293T cells. a Expression of endogenous BMPR2 by HEK293T cells compared to β-actin loading control. Approximate molecular weights are indicated. b , c BMP2 induces phosphorylation of SMAD1, 5, and 8 (pSMAD1,5,8) in HEK293T cells and this response is blunted by pre-treatment with 3F6. Approximate molecular weights are indicated in b . Results are quantified in c and expressed as mean ± SEM ratio of phosphorylated SMAD1,5,8: total SMAD1 relative to BMP2 treatment alone. No effect on BMP2-responsiveness was observed with pre-treatment using control ascites (Ctrl Asc.). n = 3 per condition. Asterisk indicates p < 0.05 by paired t test

    Techniques Used: Activation Assay, Expressing

    Antibody 3F6 has no effect on BMP-responsiveness in BMPR2 knock-down HEK293T cells. a Expression levels of endogenous BMPR2 in scramble control HEK293T cells (Control) and HEK293T cells carrying anti- BMPR2 shRNA ( BMPR2 -KD) compared to HPRT1 loading control. Data are expressed as normalized to scramble control (Control) using the 2 −∆∆Ct method. n = 6 per condition. Asterisk indicates p < 0.05 by unpaired t test. b , c Expression of endogenous BMPR2 in scramble control HEK293T cells (Control) and BMPR2 -KD HEK293T cells compared to β-actin loading control. Approximate molecular weights are indicated in b . Representative immunoblot is shown in b and results from three independent runs are quantified in c (data are expressed as mean ± SEM ratio of BMPR2: β-actin normalized to scramble control (Relative Expression)). Asterisk indicates p < 0.05 by paired t test. d , e BMP2 induces phosphorylation of SMAD1, 5, and 8 (pSMAD1,5,8) in BMPR2 - KD HEK293T cells. No effect on BMP2-responsiveness of BMPR2 - KD HEK293T cells was observed with pre-treatment using control ascites (Ctrl Asc.) or 3F6. Approximate molecular weights are indicated in d . Results are quantified in E and expressed as mean ± SEM ratio of phosphorylated SMAD1,5,8: total SMAD1 relative to BMP2 treatment alone (Relative pS1:S1). n = 4 per condition
    Figure Legend Snippet: Antibody 3F6 has no effect on BMP-responsiveness in BMPR2 knock-down HEK293T cells. a Expression levels of endogenous BMPR2 in scramble control HEK293T cells (Control) and HEK293T cells carrying anti- BMPR2 shRNA ( BMPR2 -KD) compared to HPRT1 loading control. Data are expressed as normalized to scramble control (Control) using the 2 −∆∆Ct method. n = 6 per condition. Asterisk indicates p < 0.05 by unpaired t test. b , c Expression of endogenous BMPR2 in scramble control HEK293T cells (Control) and BMPR2 -KD HEK293T cells compared to β-actin loading control. Approximate molecular weights are indicated in b . Representative immunoblot is shown in b and results from three independent runs are quantified in c (data are expressed as mean ± SEM ratio of BMPR2: β-actin normalized to scramble control (Relative Expression)). Asterisk indicates p < 0.05 by paired t test. d , e BMP2 induces phosphorylation of SMAD1, 5, and 8 (pSMAD1,5,8) in BMPR2 - KD HEK293T cells. No effect on BMP2-responsiveness of BMPR2 - KD HEK293T cells was observed with pre-treatment using control ascites (Ctrl Asc.) or 3F6. Approximate molecular weights are indicated in d . Results are quantified in E and expressed as mean ± SEM ratio of phosphorylated SMAD1,5,8: total SMAD1 relative to BMP2 treatment alone (Relative pS1:S1). n = 4 per condition

    Techniques Used: Expressing, shRNA, Western Blot

    Related Articles

    Gentle:

    Article Title: Identification of a bone morphogenetic protein type 2 receptor neutralizing antibody
    Article Snippet: .. BMPR2-ECD/Fc fusion (Sino Biologicals 10551-H03H) was mixed with 5 μL Protein G-coupled Dynabeads (Invitrogen 1003D) at room temperature for 30 min in 200 μL total volume with gentle rocking. .. The loaded beads were then washed twice with PBS and resuspended in 135 μL PBS ± 3F6 (Thermo Fisher Scientific Cat# MA5-15827) or control ascites (Sigma M8273) and mixed for 1 h at room temperature with gentle rocking.



    Similar Products

    bmpr2 ecd fc fusion  (Sino Biological)
    93
    Sino Biological bmpr2 ecd fc fusion
    Antibody 3F6 reduces <t>BMPR2-ECD</t> ligand-binding activity in a modified immunoprecipitation assay. Neutralizing the ligand-binding activity of BMPR2-ECD using various amounts of 3F6. Results are quantified by ELISA and expressed as mean ± SEM relative to the ligand-binding activity of BMPR2-ECD in the absence of 3F6. n ≥ 3 per condition. Asterisk indicates p < 0.05 by paired t test
    Bmpr2 Ecd Fc Fusion, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmpr2+ecd+fc+fusion/Human+BMPR2+%2F+BMPR-II+Protein/pmc06558810-25-0-2
    Average 93 stars, based on 1 article reviews
    bmpr2 ecd fc fusion - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    de glycosylated human bmpr2 ecd fc fusion  (R&D Systems)
    93
    R&D Systems de glycosylated human bmpr2 ecd fc fusion
    Antibody 3F6 reduces <t>BMPR2-ECD</t> ligand-binding activity in a modified immunoprecipitation assay. Neutralizing the ligand-binding activity of BMPR2-ECD using various amounts of 3F6. Results are quantified by ELISA and expressed as mean ± SEM relative to the ligand-binding activity of BMPR2-ECD in the absence of 3F6. n ≥ 3 per condition. Asterisk indicates p < 0.05 by paired t test
    De Glycosylated Human Bmpr2 Ecd Fc Fusion, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmpr2+ecd+fc+fusion/Recombinant+Human%2FMouse+BMPR-II+Fc+Chimera+Protein%2C+CF/pmc04058410-130-8-12
    Average 93 stars, based on 1 article reviews
    de glycosylated human bmpr2 ecd fc fusion - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Antibody 3F6 reduces BMPR2-ECD ligand-binding activity in a modified immunoprecipitation assay. Neutralizing the ligand-binding activity of BMPR2-ECD using various amounts of 3F6. Results are quantified by ELISA and expressed as mean ± SEM relative to the ligand-binding activity of BMPR2-ECD in the absence of 3F6. n ≥ 3 per condition. Asterisk indicates p < 0.05 by paired t test

    Journal: BMC Research Notes

    Article Title: Identification of a bone morphogenetic protein type 2 receptor neutralizing antibody

    doi: 10.1186/s13104-019-4367-0

    Figure Lengend Snippet: Antibody 3F6 reduces BMPR2-ECD ligand-binding activity in a modified immunoprecipitation assay. Neutralizing the ligand-binding activity of BMPR2-ECD using various amounts of 3F6. Results are quantified by ELISA and expressed as mean ± SEM relative to the ligand-binding activity of BMPR2-ECD in the absence of 3F6. n ≥ 3 per condition. Asterisk indicates p < 0.05 by paired t test

    Article Snippet: BMPR2-ECD/Fc fusion (Sino Biologicals 10551-H03H) was mixed with 5 µL Protein G-coupled Dynabeads (Invitrogen 1003D) at room temperature for 30 min in 200 µL total volume with gentle rocking.

    Techniques: Ligand Binding Assay, Activity Assay, Modification, Immunoprecipitation, Enzyme-linked Immunosorbent Assay

    Antibody 3F6 reduces activation of BMP pathway in HEK293T cells. a Expression of endogenous BMPR2 by HEK293T cells compared to β-actin loading control. Approximate molecular weights are indicated. b , c BMP2 induces phosphorylation of SMAD1, 5, and 8 (pSMAD1,5,8) in HEK293T cells and this response is blunted by pre-treatment with 3F6. Approximate molecular weights are indicated in b . Results are quantified in c and expressed as mean ± SEM ratio of phosphorylated SMAD1,5,8: total SMAD1 relative to BMP2 treatment alone. No effect on BMP2-responsiveness was observed with pre-treatment using control ascites (Ctrl Asc.). n = 3 per condition. Asterisk indicates p < 0.05 by paired t test

    Journal: BMC Research Notes

    Article Title: Identification of a bone morphogenetic protein type 2 receptor neutralizing antibody

    doi: 10.1186/s13104-019-4367-0

    Figure Lengend Snippet: Antibody 3F6 reduces activation of BMP pathway in HEK293T cells. a Expression of endogenous BMPR2 by HEK293T cells compared to β-actin loading control. Approximate molecular weights are indicated. b , c BMP2 induces phosphorylation of SMAD1, 5, and 8 (pSMAD1,5,8) in HEK293T cells and this response is blunted by pre-treatment with 3F6. Approximate molecular weights are indicated in b . Results are quantified in c and expressed as mean ± SEM ratio of phosphorylated SMAD1,5,8: total SMAD1 relative to BMP2 treatment alone. No effect on BMP2-responsiveness was observed with pre-treatment using control ascites (Ctrl Asc.). n = 3 per condition. Asterisk indicates p < 0.05 by paired t test

    Article Snippet: BMPR2-ECD/Fc fusion (Sino Biologicals 10551-H03H) was mixed with 5 µL Protein G-coupled Dynabeads (Invitrogen 1003D) at room temperature for 30 min in 200 µL total volume with gentle rocking.

    Techniques: Activation Assay, Expressing

    Antibody 3F6 has no effect on BMP-responsiveness in BMPR2 knock-down HEK293T cells. a Expression levels of endogenous BMPR2 in scramble control HEK293T cells (Control) and HEK293T cells carrying anti- BMPR2 shRNA ( BMPR2 -KD) compared to HPRT1 loading control. Data are expressed as normalized to scramble control (Control) using the 2 −∆∆Ct method. n = 6 per condition. Asterisk indicates p < 0.05 by unpaired t test. b , c Expression of endogenous BMPR2 in scramble control HEK293T cells (Control) and BMPR2 -KD HEK293T cells compared to β-actin loading control. Approximate molecular weights are indicated in b . Representative immunoblot is shown in b and results from three independent runs are quantified in c (data are expressed as mean ± SEM ratio of BMPR2: β-actin normalized to scramble control (Relative Expression)). Asterisk indicates p < 0.05 by paired t test. d , e BMP2 induces phosphorylation of SMAD1, 5, and 8 (pSMAD1,5,8) in BMPR2 - KD HEK293T cells. No effect on BMP2-responsiveness of BMPR2 - KD HEK293T cells was observed with pre-treatment using control ascites (Ctrl Asc.) or 3F6. Approximate molecular weights are indicated in d . Results are quantified in E and expressed as mean ± SEM ratio of phosphorylated SMAD1,5,8: total SMAD1 relative to BMP2 treatment alone (Relative pS1:S1). n = 4 per condition

    Journal: BMC Research Notes

    Article Title: Identification of a bone morphogenetic protein type 2 receptor neutralizing antibody

    doi: 10.1186/s13104-019-4367-0

    Figure Lengend Snippet: Antibody 3F6 has no effect on BMP-responsiveness in BMPR2 knock-down HEK293T cells. a Expression levels of endogenous BMPR2 in scramble control HEK293T cells (Control) and HEK293T cells carrying anti- BMPR2 shRNA ( BMPR2 -KD) compared to HPRT1 loading control. Data are expressed as normalized to scramble control (Control) using the 2 −∆∆Ct method. n = 6 per condition. Asterisk indicates p < 0.05 by unpaired t test. b , c Expression of endogenous BMPR2 in scramble control HEK293T cells (Control) and BMPR2 -KD HEK293T cells compared to β-actin loading control. Approximate molecular weights are indicated in b . Representative immunoblot is shown in b and results from three independent runs are quantified in c (data are expressed as mean ± SEM ratio of BMPR2: β-actin normalized to scramble control (Relative Expression)). Asterisk indicates p < 0.05 by paired t test. d , e BMP2 induces phosphorylation of SMAD1, 5, and 8 (pSMAD1,5,8) in BMPR2 - KD HEK293T cells. No effect on BMP2-responsiveness of BMPR2 - KD HEK293T cells was observed with pre-treatment using control ascites (Ctrl Asc.) or 3F6. Approximate molecular weights are indicated in d . Results are quantified in E and expressed as mean ± SEM ratio of phosphorylated SMAD1,5,8: total SMAD1 relative to BMP2 treatment alone (Relative pS1:S1). n = 4 per condition

    Article Snippet: BMPR2-ECD/Fc fusion (Sino Biologicals 10551-H03H) was mixed with 5 µL Protein G-coupled Dynabeads (Invitrogen 1003D) at room temperature for 30 min in 200 µL total volume with gentle rocking.

    Techniques: Expressing, shRNA, Western Blot